A de novo Transcriptome Assembly of the European Flounder (Platichthys flesus): The Preselection of Transcripts Encoding Active Forms of Enzymes

The RNA sequencing data sets available for different fish species show a potentially high variety of forms enzymes just in teleosts. This is primarily considered an effect the first round whole-genome duplication with mutations duplicated genes (isozymes) and alternative splicing mRNA (isoforms). However, abundance transcript variants not necessarily reflected active proteins. We have investigated transcriptional profiles two enzymes, aralkylamine N-acetyltransferase (AANAT: EC 2.3.1.87) N-acetylserotonin O-methyltransferase (ASMT: 2.1.1.4), eyeball, brain, intestines, spleen, heart, liver, head kidney, gonads, skin European flounder ( Platichthys flesus ). High-throughput next-generation technology NovaSeq6000 was used to generate 500M reads. These were then assembled filtered producing 75k reliable contigs. Gene ontology (GO) terms assigned majority annotated contigs/unigenes based on results PFAM, PANTHER, UniProt, InterPro protein database searches. BUSCOs statistics metazoa, vertebrata, actinopterygii databases showed that reported transcriptome represents level completeness. In this article, we how preselect transcripts encoding (isozymes or isoforms), using AANAT ASMT as examples. can be tool design experiments well basis discussion diversity enzyme their physiological relevance